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Electrostimulation induces cardiomyocyte predifferentiation of fibroblasts.

Electrostimulation induces cardiomyocyte predifferentiation of fibroblasts.

Genovese JA, Spadaccio C, Langer J, Habe J, Jackson J, Patel AN

Abstract:

Stem-cell therapy has become a promising therapeutic tool for myocardial repair. Cardiac pre-committed
cells, which complete their differentiation in the myocardium, may reduce fibrosis and restore muscle
function. However, many questions concerning a precise, functional integration of injected cells remain
unanswered. Fibroblasts regulate the cardiac extracellular matrix and are the most abundant cell population
in an infarcted area. Electrostimulation is a well-known trophic factor and can induce phenotypic
changes in myoblasts. The objective of this study was to evaluate the effectiveness of electrical stimulation
to induce pre-commitment of fibroblasts into cardiomyocytes in vitro. Using short-time electrostimulation
in a cytokine-free culture system, we induced pre-commitment of two fibroblast cell lines to
a cardiomyocyte phenotype. This partial differentiation in vitro may facilitate further differentiation
within the cardiac environment and result in better electro-mechanical integration of the therapeutically
introduced cells.

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