Activation of Epidermal Vanilloid Receptor-1 Induces Release of Proinflammatory Mediators in Human Keratinocytes

Author(s) Michael D. Southall, Tao Li, Lera S. Gharibova, Yong Pei, Grant D. Nicol, and Jeffrey B. Travers
Journal J. Pharmacol. Exp. Ther., 304

Method Extract

... ...could then be superfused with Ringers solution. The Fura-2 fluorescence at 340 and 380 nm was measured with an IonOptix recording system (IonOptix, Milton, MA) and changes in calcium were expressed as the ratio of f340/f380. In a separate series of experiments... ...

Keywords keratinocytes, calcium imaging, Fura2
Our Ref: 1216