Electrostimulation induces cardiomyocyte predifferentiation of fibroblasts
| Author(s) | Jorge A. Genovese a,Cristiano Spadaccio, Jason Langer, Jaclyn Habe, Johnna Jackson, Amit N. Patel |
| Journal | 370 (2008) 450–455 |
Method Extract | |
| c-pace | |
Abstract | |
| Stem-cell therapy has become a promising therapeutic tool for myocardial repair. Cardiac pre-committed cells, which complete their differentiation in the myocardium, may reduce fibrosis and restore muscle function. However, many questions concerning a precise, functional integration of injected cells remain unanswered. Fibroblasts regulate the cardiac extracellular matrix and are the most abundant cell population in an infarcted area. Electrostimulation is a well-known trophic factor and can induce phenotypic changes in myoblasts. The objective of this study was to evaluate the effectiveness of electrical stimulation to induce pre-commitment of fibroblasts into cardiomyocytes in vitro. Using short-time electrostimulation in a cytokine-free culture system, we induced pre-commitment of two fibroblast cell lines to a cardiomyocyte phenotype. This partial differentiation in vitro may facilitate further differentiation within the cardiac environment and result in better electro-mechanical integration of the therapeutically introduced cells. | |
| Keywords | fibroblasts, myotubes, c-pace, cardiac |
Our Ref: 1615