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This protocol describes the isolation of primary adult mouse cardiomyocytes using sequential calcium depletion, enzymatic digestion, and gradual calcium reintroduction. The approach is adapted from the method of Matthew Ackers-Johnson et al.
User's guide for the Intact Muscle System.
KoForce force calibration worksheet
Work Loop Power Analysis: Mechanical work in myocardial slices
Action Potential Measurements in iPSC-derived and Primary Cardiomyocytes
User's guide for the CytoCypher MultiCell System.
In this virtual demo, Drs. Adam Veteto and Hanan Qasim show how to successfully acquire force, resting sarcomere length, and calcium data from cardiac slices, including tips and best practices.
Drs. Bradley Palmer, Matthew Caporizzo, and Benjamin Lee share how the IonOptix Cardiac Slice System can be used to complement and improve upon traditional cardiac investigations.
Calcium Indicator Dye Loading in Cardiac Slices
