Lying between single cell and whole heart experimental studies, intact papillary muscle isolated from hearts offers unique advantages. Unlike single isolated myocytes, which represent the smallest fully functional model system, intact papillary muscle can be used to study cardiac function within a multicellular context and an intact 3-dimensional myofilament lattice. And unlike whole heart studies, the contractile characteristics of papillary muscle can be evaluated independently of extrinsic factors such as vascular tone. Papillary muscle also allows measurements too difficult or impossible to perform in whole hearts. Similarly, unlike skinned preparations, intact muscle preparations allow for simultaneous detection of force production and intracellular calcium dynamics.

IonOptix offers a turn-key solution for studying isolated muscle tissue…

IonOptix Intact Muscle Chamber System


The IonOptix Intact Muscle Chamber facilitates simultaneous measurement of quantitative fluorescence and force development. Intact ventricular papillary muscle or other excitable muscle tissue is attached between a robust force transducer and programmable length controller. The chamber is designed to be used for excitable muscles up to 2 cm in length. Chamber fluid flow allows for temperature control and continuous oxygenation of tissue, while the specialized force transducer also permits electrical excitation directly through the platinum hooks attached to the muscle preparation, ensuring no electrolysis or disruption of biology. LEARN MORE

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