|IonOptix Video Tutorials: Operation of the C-Pace EM Culture Stimulator|| Culture Pacing||Tutorials, Video||C-Dish, C-Pace EM, C-Stretch|
A video tutorial demonstrating operation of the IonOptix C-Pace EM culture stimulator.
|Short video: Cardiac Slice Work Loop_4 Hz|| Intact Muscle Chamber||Video||Intact Muscle Chamber|
A short video showing a mouse cardiac slice performing work loops at 4 Hz.
|Short video: Cardiac Slice Work Loop_2 Hz|| Intact Muscle Chamber||Video||Intact Muscle Chamber|
An example of a rat cardiac slice undergoing a force-length work loop, which is analogous to the pressure volume work loop of the whole heart.
|Short video: Cardiac Slice Work Loop_0.5 Hz|| Intact Muscle Chamber||Video||Intact Muscle Chamber|
This short video showug a rat cardiac slice muscle undergoing a work loop generated using force feedback.
|Preparation of a cardiac slice|| Intact Muscle Chamber||Tutorials, Video||Intact Muscle Chamber|
A video tutorial demonstrating cardiac slice preparation.
|Functional Characterization of Excitation-Contraction Coupling in Cardiomyocytes|| Calcium and Contractility, MultiCell HTS||Video, Webinars||LED Light Source, PMT Sub-System|
Dr. Joe Soughayer and Dr. Adam Veteto provide an in-depth overview of excitation-contraction (E-C) coupling in cardiomyocytes and the tools to characterize this.
|Excitation-Contraction Coupling: Simultaneous Measures of Intracellular Calcium & Action Potentials|| Calcium and Contractility, MultiCell HTS||Video, Webinars||LED Light Source|
Patrick Schönleitner, PhD and Francisco Altamirano, PhD present their work involving simultaneous measurements of intracellular calcium, membrane voltage, and contractility of cardiomyocytes.
|Measuring iPSC-CM Calcium & Contractility|| Calcium and Contractility, MultiCell HTS||Application & Tech Notes|
Measuring hiPSC-CM Ca & Contractility
|iPSC-derived Cardiomyocyte Contractility Acquisition and Analysis Demo|| Calcium and Contractility, MultiCell HTS||Video, Webinars|
Understand how to acquire and analyze contractility data from iPSC-derived cardiomyocytes.
|A How-To Demonstration of Higher Throughput Excitation-Contraction Coupling Investigations|| MultiCell HTS||Video, Webinars|
From myocyte isolation to data acquisition, analysis, and post-analysis plotting, Dr. Michiel Helmes and Dr. Diederik Kuster demonstrate best practices and new techniques in high-content, higher throughput investigations of excitation-contraction coupling in isolated cardiomyocytes.
|Simultaneous Action Potential, Calcium, and Contractility Case Study|| Calcium and Contractility, MultiCell HTS||Application & Tech Notes||LED Light Source, PMT Sub-System|
Combined OAP, Ca, and Cont measurements in adult cardiomyocytes
|Removal of papillary muscles from a rat heart|| Intact Muscle Chamber||Tutorials, Video||Intact Muscle Chamber|
A video tutorial demonstrating papillary muscle isolation from a rat heart.
|Cairn OptoLED Manual|| Calcium and Contractility, MultiCell HTS||User Manuals||LED Light Source|
|Intact Muscle Chamber Manual|| Intact Muscle Chamber||User Manuals||Intact Muscle Chamber|
|IonOptix Video Tutorials: Murine Cardiomyocyte Isolation|| Calcium and Contractility, Culture Pacing, MultiCell HTS, MyoStretcher||Tutorials, Video||Cell Framing Adapter, CytoCypher Perfusion, FHD Microscope Chamber, HyperSwitch Light Source, LED Light Source, MyoCam-S3, MyoPacer Cell Stimulator, MyoPacer EP Cell Stimulator, MyoTak, OptiForce, PMT Sub-System, µStep Light Source|
A video tutorial highlighting murine cardiomyocyte isolation protocol and techniques.
|MultiCell Lite Case Study|| MultiCell HTS||Application & Tech Notes|
|IonOptix Video Tutorials: IonWizard Experiment Design|| Calcium and Contractility||Tutorials, Video||Cell Framing Adapter, FHD Microscope Chamber, HyperSwitch Light Source, LED Light Source, MyoCam-S3, MyoPacer Cell Stimulator, MyoPacer EP Cell Stimulator, PMT Sub-System, µStep Light Source|
A video tutorial highlighting experiment design in IonWizard.
|IonOptix Video Tutorials: Calcium & Contractility System Acquisition Quick Start|| Calcium and Contractility||Tutorials, Video||Cell Framing Adapter, FHD Microscope Chamber, HyperSwitch Light Source, LED Light Source, MyoCam-S3, MyoPacer Cell Stimulator, MyoPacer EP Cell Stimulator, PMT Sub-System, µStep Light Source|
A video tutorial highlighting best practices for data acquisition of calcium and contractility data.
|IonOptix Video Tutorials: CytoSolver Batch Analysis|| Calcium and Contractility, MultiCell HTS||Tutorials, Video||Cell Framing Adapter, HyperSwitch Light Source, LED Light Source, Motic AE31, MyoCam-S3, MyoPacer Cell Stimulator, MyoPacer EP Cell Stimulator, PMT Sub-System|
A simple video tutorial highlighting analysis of calcium and contractility data using IonWizard.
|IonOptix Video Tutorials: Analyzing Unmarked Events|| Calcium and Contractility||Tutorials, Video||Cell Framing Adapter, Cell Microcontrols mTCII, FHD Microscope Chamber, HyperSwitch Light Source, LED Light Source, Motic AE31, MyoCam-S3, MyoPacer Cell Stimulator, MyoPacer EP Cell Stimulator, Olympus IX73, PMT Sub-System, µStep Light Source|
A simple video tutorial highlighting analysis of calcium and contractility data using IonWizard.
|IonOptix Video Tutorials: IonWizard Data Analysis|| Calcium and Contractility||Tutorials, Video||Cell Framing Adapter, Cell Microcontrols mTCII, FHD Microscope Chamber, HyperSwitch Light Source, LED Light Source, Motic AE31, MyoCam-S3, MyoPacer Cell Stimulator, MyoPacer EP Cell Stimulator, Olympus IX73, PMT Sub-System, µStep Light Source|
A simple video tutorial highlighting analysis of calcium and contractility data using IonWizard.
|CytoCypher Perfusion Manual|| MultiCell HTS||User Manuals||CytoCypher Perfusion|
|Hypertrophic cardiomyopathy in high-fat diet-induced obesity: role of suppression of forkhead transcription factor and atrophy gene transcription.|| Calcium and Contractility||Publication Reviews||HyperSwitch Light Source, MyoPacer Cell Stimulator|
Fang, C. X., Dong, F., Thomas, D. P., Ma, H., He, L., & Ren, J. (2008). American Journal of Physiology-Heart and Circulatory Physiology, 295(3)
|FHD Microscope Chamber Specifications||Brochures & Handouts||FHD Microscope Chamber|
|Vessel System HyperSwitch Config|| Blood Vessel||Brochures & Handouts||HyperSwitch Light Source|
|Vessel System Brochure|| Blood Vessel||Brochures & Handouts|
|MultiCell Lite Handout|| MultiCell HTS||Brochures & Handouts|
|MultiCell Handout|| MultiCell HTS||Brochures & Handouts|
|MyoStretcher Handout|| MyoStretcher||Brochures & Handouts|
|Workloops Handout|| MyoStretcher||Brochures & Handouts|
|C-Pace EM Brochure|| Culture Pacing||Brochures & Handouts||C-Pace EM|
|Motic AE31 Manual||User Manuals||Motic AE31|
|MyoTak Handout||Brochures & Handouts||MyoTak|
|Intact Muscle Chamber Handout|| Intact Muscle Chamber||Brochures & Handouts||Intact Muscle Chamber|
|Setting Up An Experiment In IW6||Application & Tech Notes|
This document explains how to set up the software to run an experiment.
|Hardware Configuration IW6 With IO24 And Mutech Cards|| Blood Vessel, Calcium and Contractility||Application & Tech Notes|
This document contains a step-by-step guide for the hardware configuration of IonWizard 6 under windows XP.
|How To Upgrade To A New Version Of IW6||Application & Tech Notes|
These documents explains how to upgrade from an existing version to a newer version of IonWizard 6.
|Transient Analysis Features||Application & Tech Notes|
The following document provides a full description of the mathematical fit of the data followed by a description of each of the analysis results and their application.
|Mutech And IO24 Card Installation For IW6||Application & Tech Notes|
This document contains a step by step guide for the installation of the new IO24 PCI card and Mutech video card used for contractility and diameter measurements.
|SarcLen & FFTs||Application & Tech Notes|
Applying a Fast Fourier Transform to Sarcomere Length Measurements
|Plotting Calcium And Contractility||Application & Tech Notes|
The following is a Excel spreadsheet with a macro enabling the interpolation of data exported from IonWizard.
|Plotting Hysteresis Loops||Application & Tech Notes|
The following document details a procedure for plotting both calcium and contracility
|SarcLen Algorithm||Application & Tech Notes|
The following document describes the application of the fast Fourier transform to sarcomere length using IonOptix SarcLen.
|Testing MyoPacer Outputs||Application & Tech Notes||MyoPacer Cell Stimulator|
This tech note describes how to use a standard volt meter to evaluate that the high voltage output of your myopacer is functioning properly.
|Replacing Your Xe Bulb||Application & Tech Notes||HyperSwitch Light Source, µStep Light Source|
A brief guide to properly replacing your Xenon bulb with plenty of helpful pictures.
|Changing Well Number||Application & Tech Notes||C-Dish, C-Pace EM|
This is a short note on changing the number of wells that the C-Pace or C-Pace EP will stimulate.
|Adding HV Boards To C-Pace||Application & Tech Notes||C-Dish, C-Pace EM|
This manual illustrates the steps necessary to add High Voltage Boards to a C-Pace.
|Adding HV Boards To C-Pace EP||Application & Tech Notes||C-Dish, C-Pace EM|
This manual illustrates the steps necessary to add High Voltage Boards to a C-Pace EP.
|IonOptix Kiosk||Application & Tech Notes|
This zip file contains a short kiosk-style presentation (self-advancing). It gives a general outline of the components assembled in the IonOptix Myocyte Recording System.
|Vessel Flow Characteristics – Unit Conversions|| Blood Vessel||Application & Tech Notes|
The following document offers a unit conversion example for our FloAcq vessel flow characteristics software acquisition module.
|Mutech and RTD card installation for IW6|| Calcium and Contractility||Application & Tech Notes|
This document contains a step by step guide for the installation of the Mutech video card used for contractility and diameter measurements.
|Myocyte Calcium & Contractility Recording System (µStep Configuration)|| Calcium and Contractility||Brochures & Handouts||µStep Light Source|
Complete real-time, turnkey system for calcium and contractility recording
|Myocyte Calcium & Contractility Recording System (HyperSwitch Configuration)|| Calcium and Contractility||Brochures & Handouts||HyperSwitch Light Source|
Complete real-time, turnkey system for calcium and contractility recording
|Calcium And Contractility||Application & Tech Notes|
Record Calcium & Contractility from Intact Cardiomyocytes
|InsideAnswers: Techniques and Best Practices for Cardiomyocyte Isolation|| Calcium and Contractility, MultiCell HTS, MyoStretcher||Video, Webinars|
In this Q&A video series, experts address your best questions and greatest challenges with regards to cardiomyocyte isolation, purification, culture and analysis.
|µStep Hardware Manual||User Manuals||µStep Light Source|
|Photon-to-Voltage User’s Tech Note||User Manuals||Photon-To-Voltage Converter|
|OptiForce Manual||User Manuals||OptiForce|
|MyoPacer Manual||User Manuals||MyoPacer Cell Stimulator|
|MyoPacer EP Manual||User Manuals||MyoPacer Cell Stimulator, MyoPacer EP Cell Stimulator|
|MyoCam-S Manual||User Manuals||MyoCam-S3|
|IonWizard Core and Analysis Manual||User Manuals|
|IonWizard Acquisition Manual||User Manuals|
|HyperSwitch Hardware Manual||User Manuals||HyperSwitch Light Source|
|FluoroPlex Manual||User Manuals|
|CFA Hardware Manual||User Manuals||Cell Framing Adapter|
|C-Stretch Manual||User Manuals||C-Stretch|
|C-Pace Navigator Manual||User Manuals||C-Pace EM|
|C-Pace EM Manual|| Culture Pacing||User Manuals||C-Pace EM|
|C-Dish Manual|| Culture Pacing||User Manuals||C-Dish, C-Pace EM|
|Analyzing Unmarked Events||Tutorials|
In order for IonWizard to fit untriggered events, you need to tell the software where the event begins, or, tell the software to look for these events for you. This document explains how to do this.
This document details calibration of an IonOptix system equipped for measuring calcium using Fura-2.
|Spatial Calibration Using The Sarc Tool||Tutorials|
This documents details the procedure for spatially calibrating an IonOptix system using the Sarcomere Tool.
|Transient Analysis Tutor||Tutorials|
This document shows in steps how to do a standard analysis with IonWizard 5 & 6. We have also added an example data file that you can use to practice the tutorial with.
|Vessel Flow Characterization|| Blood Vessel||Application & Tech Notes|
The endothelial response to flow-induced stress (e.g., the generation and secretion of nitric oxide) is an important modulator of vascular tone and function, linking vasodilation and constriction to …
|Cardiac Myocytes|| Calcium and Contractility||Application & Tech Notes|
The largest group of IonOptix users are investigators who study cardiac myocytes and want information about contractility parameters and the associated calcium transients. Our aim is to offer you a complete recording system for these experiments.
|Benefits Of Chronic Pacing Of Cells In Culture|| Culture Pacing||Application & Tech Notes||C-Pace EM|
Chronic electrical stimulation has been shown to prevent the dedifferentiation of myocytes that occurs in long term culture…
|Applications For The C-Pace System|| Culture Pacing||Application & Tech Notes||C-Pace EM|
5 years of the C-Pace: a short review Cardiac tissue * Skeletal muscle * Stem cells * Cell-lines * Cardiogenic Fibroblasts * Neuronal fibroblasts * Osteoblasts It has been more than 5 years …
|Interpolated Numerator Method|| Calcium and Contractility||Application & Tech Notes|
For dual excitation measurements with Fura-2, we offer two methods of collecting data, interleaved sampling and the interpolated numerator (or denominator) method.
|Basic Principles Of Fluorescence And Ion Sensing|| Calcium and Contractility||Application & Tech Notes|
Introduction This is a very, very basic introduction into the theory of fluorescence measurements and its practical implications as applied to experiments done using IonOptix equipment. For a thorough …
|Loading Fura-2 into cardiomyocytes||Application & Tech Notes|
Many different protocols exist for loading cells with dye, due to diffences between cell types in how they treat Fura. Below a protocol is provided that works well for freshly isolated cardiac …
|Optimizing Fluorescence Signal Quality|| Calcium and Contractility||Application & Tech Notes|
Overview Fluorescence signal quality is directly proportional to fluorescence light intensity (which is proportional to excitation light intensity, fluorescence yield of the fluorophore, and light …
|Culture Pacing|| Culture Pacing||Application & Tech Notes||C-Pace EM|
Engineered skeletal muscle from electrically stimulated myoblast cells Check out this video from researchers at the Department of Biomedical Engineering, Eindhoven University of Technology.
|Calcium and cell length studies in adult cardiac myocytes|| Calcium and Contractility||Application & Tech Notes|
Check out this video from researchers at the Department of Internal Medicine B, University Medicine Greifswald.
|Pressurized Blood Vessel|| Blood Vessel||Application & Tech Notes||VesCam|
Interest in the physiology and pathology of the circulatory system has led to a substantial increase in the number of laboratories studying isolated and pressurized blood vessels.
|G protein-coupled receptor kinase 2 ablation in cardiac myocytes before or after myocardial infarction prevents heart failure.|| Calcium and Contractility||Publication Reviews||HyperSwitch Light Source|
Raake, P. W., Vinge, L. E., Gao, E., Boucher, M., Rengo, G., Chen, X., DeGeorge Jr., B.R., Matkovich, S., Houser, S.R., Most, P., Eckhart, A.D., Dornll, G.W., and Koch, W. J. (2008). Circulation Research, 103(4), 413-422
|Electro-mechanical conditioning of human iPSC-derived cardiomyocytes for translational research||Publication Reviews||C-Stretch|
Kroll, K., Chabria, M., Wang, K., Häusermann, F., Schuler, F., & Polonchuk, L. (2017). Progress in Biophysics and Molecular Biology, 130, 212-222
|Measuring Force in Single Heart Cells||Video, Webinars|
New technology now provides investigators the ability to reliably attach myocytes, mechanically stretch them, make direct force measurements and control cell length intelligently. This webinar reviews best-practices and techniques for attaching, stretching, and studying isolated cells.
|Measuring Work in Single Isolated Cardiomyocytes: Replicating the Cardiac Cycle||Video, Webinars|
A special webinar for basic cardiovascular researchers interested in a novel technique for measuring work output and replicating the four phases of the cardiac cycle at the single cell level.
|Techniques and Best Practices for Cardiomyocyte Isolation|| Calcium and Contractility, MultiCell HTS, MyoStretcher||Video, Webinars||C-Stim CMC Microscope Chamber, Cell Framing Adapter, Cell Microcontrols mTCII, CytoCypher Perfusion, FHD Microscope Chamber, HyperSwitch Light Source, LED Light Source, Motic AE31, MyoCam-S3, MyoPacer Cell Stimulator, MyoPacer EP Cell Stimulator, MyoTak, Olympus IX73, OptiForce, PMT Sub-System, µStep Light Source|
Ronglih Liao and Davor Pavlovic discuss techniques for isolating myocytes using both traditional and Langendorff-free methodologies.
|Glucocorticoid- and protein kinase A-dependent transcriptome regulation in airway smooth muscle.||Publication Reviews||HyperSwitch Light Source|
Misior, A. M., Deshpande, D. A., Loza, M. J., Pascual, R. M., Hipp, J. D., & Penn, R. B. (2009). American Journal of Respiratory Cell and Molecular Biology, 41(1), 24-39
|High Throughput Investigation of EC Coupling in Isolated Cardiac Myocytes||Video, Webinars|
Michiel Helmes, PhD presents new technology and methodology for fast data acquisition and functional analysis of cardiac myocytes, leading to better lab practices and data interpretation.
|Breaking sarcomeres by in vitro exercise.||Publication Reviews||C-Pace EM|
Orfanos, Z., Gödderz, M. P., Soroka, E., Gödderz, T., Rumyantseva, A., van der Ven, P.F.M, Hawke, T.J., Fürst, D. O. (2016). Scientific Reports, 6(1)
|Overexpression of FK506-Binding Protein FKBP12.6 in Cardiomyocytes Reduces Ryanodine ReceptorMediated Ca2+ Leak From the Sarcoplasmic Reticulum and Increases Contractility|
|Myocyte contractile activity modulates norepinephrine cytotoxicity and survival effects of neuregulin-1beta.|
Myocyte contractile activity modulates norepinephrine cytotoxicity and survival effects of neuregulin-1beta. Kuramochi Y1, Lim CC, Guo X, Colucci WS, Liao R, Sawyer DB Abstract: The purpose of this study is to test the hypothesis that mechanical and electrical activity in adult rat ventricular myocytes (ARVM) alters responses to proapoptotic and prosurvival ligands. The effects of electrical stimulation on myocyte survival, stress signaling, response to beta-adrenergic receptor (beta-AR)-stimulated apoptosis, and neuregulin-1beta […]
|Intraluminal Pressure Is a Stimulus for NFATc3 Nuclear Accumulation: ROLE OF CALCIUM, ENDOTHELIUM-DERIVED NITRIC OXIDE, AND cGMP-DEPENDENT PROTEIN KINASE|| Blood Vessel|
The transcription factor NFAT (nuclear factor of activated T-cells) is implicated in cardiac hypertrophy and vasculogenesis. NFAT activation, reflecting dephosphorylation by the calcium-dependent phosphatase, calcineurin, and subsequent nuclear localization, is generally thought to require a sustained increase in intracellular calcium. However, in smooth muscle we have found that elevation of calcium by membrane depolarization fails to induce an increase in nuclear localization of the NFATc3 isoform. Here, we demonstrate that […]
|Rapid electrical stimulation induces early activation of kinase signal transduction pathways and apoptosis in adult rat ventricular myocytes.|| Culture Pacing||C-Dish|
Rapid electrical stimulation induces early activation of kinase signal transduction pathways and apoptosis in adult rat ventricular myocytes. Kuramochi Y1, Guo X, Sawyer DB, Lim CC Abstract: Chronic tachycardia in patients and rapid pacing in animal models induce myocardial dysfunction and initiate a cascade of compensatory adaptations that are ultimately unsustainable, leading to ventricular enlargement and failure. The molecular pathogenesis during the early stages of tachycardia-induced cardiomyopathy, however, remains unclear. […]
|Induction of heat shock response protects the heart against atrial fibrillation.|| Culture Pacing||C-Pace EM|
Induction of heat shock response protects the heart against atrial fibrillation. Brundel BJ, Shiroshita-Takeshita A, Qi X, Yeh YH, Chartier D, van Gelder IC, Henning RH, Kampinga HH, Nattel S Abstract: There is evidence suggesting that heat shock proteins (HSPs) may protect against clinical atrial fibrillation (AF). We evaluated the effect of HSP induction in an in vitro atrial cell line (HL-1) model of tachycardia remodeling and in tachypaced isolated […]
|Myogenic tone, reactivity, and forced dilatation: a three-phase model of in vitro arterial myogenic behavior|| Blood Vessel|
Myogenic behavior, prevalent in resistance arteries and arterioles, involves arterial constriction in response to intravascular pressure. This process is often studied in vitro by using cannulated, pressurized arterial segments from different regional circulations. We propose a comprehensive model for myogenicity that consists of three interrelated but dissociable phases: 1) the initial development of myogenic tone (MT), 2) myogenic reactivity to subsequent changes in pressure (MR), and 3) forced dilatation at […]
|Overexpression of Na+/Ca2+ exchanger alters contractility and SR Ca2+ content in adult rat myocytes.|| Culture Pacing|
Overexpression of Na+/Ca2+ exchanger alters contractility and SR Ca2+ content in adult rat myocytes. Zhang XQ, Song J, Rothblum LI, Lun M, Wang X, Ding F, Dunn J, Lytton J, McDermott PJ, Cheung JY Abstract: The functional consequences of overexpression of rat heart Na+/Ca2+ exchanger (NCX1) were investigated in adult rat myocytes in primary culture. When maintained under continued electrical field stimulation conditions, cultured adult rat myocytes retained normal contractile […]
|High-fat Diet-induced Obesity Leads to Resistance to Leptin-induced Cardiomyocyte Contractile Response.|| Calcium and Contractility|
High-fat Diet-induced Obesity Leads to Resistance to Leptin-induced Cardiomyocyte Contractile Response. Jun Ren, Bang-Hao Zhu, David P. Relling, Lucy B. Esberg, Asli F. Ceylan-Isik Abstract: Levels of the obese gene product leptin are often elevated in obesity and may contribute to obesity-induced cardiovascular complications. However, the role of leptin in obesity-associated cardiac abnormalities has not been clearly defined. This study was designed to determine the influence of high-fat diet-induced obesity […]
|The control of Ca2+ influx and NFATc3 signaling in arterial smooth muscle during hypertension.|
The control of Ca2+ influx and NFATc3 signaling in arterial smooth muscle during hypertension. Nieves-Cintrón M, Amberg GC, Navedo MF, Molkentin JD, Santana LF Abstract: Many excitable cells express L-type Ca2+ channels (LTCCs), which participate in physiological and pathophysiological processes ranging from memory, secretion, and contraction to epilepsy, heart failure, and hypertension. Clusters of LTCCs can operate in a PKCα-dependent, high open probability mode that generates sites of sustained Ca2+ […]
|Type 1 inositol 1,4,5-trisphosphate receptors mediate UTP-induced cation currents, Ca2+ signals, and vasoconstriction in cerebral arteries.|| Blood Vessel||HyperSwitch Light Source|
Type 1 inositol 1,4,5-trisphosphate receptors mediate UTP-induced cation currents, Ca2+ signals, and vasoconstriction in cerebral arteries. Zhao G, Adebiyi A, Blaskova E, Xi Q, Jaggar JH Abstract: Inositol 1, 4, 5-trisphosphate receptors (IP3Rs) regulate diverse physiological functions, including contraction and proliferation. Three IP3R isoforms exist, but functional significance of these subtypes in arterial smooth muscle cells is unclear. Here, we investigated relative expression and physiological functions of IP3R isoforms in […]
|Role of the transcription factor sox4 in insulin secretion and impaired glucose tolerance.||HyperSwitch Light Source|
Role of the transcription factor sox4 in insulin secretion and impaired glucose tolerance. Goldsworthy M, Hugill A, Freeman H, Horner E, Shimomura K, Bogani D, Pieles G, Mijat V, Arkell R, Bhattacharya S, Ashcroft FM, Cox RD Abstract: OBJECTIVES— To identify, map, clone, and functionally validate a novel mouse model for impaired glucose tolerance and insulin secretion. RESEARCH DESIGN AND METHODS— Haploinsufficiency of the insulin receptor and associated mild insulin […]
|Reactive oxygen species mediate RhoA/Rho kinase-induced Ca2+ sensitization in pulmonary vascular smooth muscle following chronic hypoxia.|| Blood Vessel||HyperSwitch Light Source|
Reactive oxygen species mediate RhoA/Rho kinase-induced Ca2+ sensitization in pulmonary vascular smooth muscle following chronic hypoxia. Jernigan NL, Walker BR, Resta TC Abstract: Recent evidence supports a prominent role for Rho kinase (ROK)-mediated pulmonary vasoconstriction in the development and maintenance of chronic hypoxia (CH)-induced pulmonary hypertension. Endothelin (ET)-1 contributes to the pulmonary hypertensive response to CH and recent studies by our laboratory and others indicate that pulmonary vascular reactivity following […]
|Novel role of calpain-3 in the triad-associated protein complex regulating calcium release in skeletal muscle.||HyperSwitch Light Source|
Novel role of calpain-3 in the triad-associated protein complex regulating calcium release in skeletal muscle. Kramerova I, Kudryashova E, Wu B, Ottenheijm C, Granzier H, Spencer MJ Abstract: Calpain-3 (CAPN3) is a non-lysosomal cysteine protease that is necessary for normal muscle function, as mutations in CAPN3 result in an autosomal recessive form of limb girdle muscular dystrophy type 2A. To elucidate the biological roles of CAPN3 in skeletal muscle, we […]
|Phosphorylation of phospholamban at threonine-17 reduces cardiac adrenergic contractile responsiveness in chronic pressure overload-induced hypertrophy.|| Culture Pacing||C-Dish|
Phosphorylation of phospholamban at threonine-17 reduces cardiac adrenergic contractile responsiveness in chronic pressure overload-induced hypertrophy. Mills GD, Kubo H, Harris DM, Berretta RM, Piacentino V 3rd, Houser SR Abstract: Physiological hemodynamic stress, such as aerobic exercise, is intermittent and requires an increase in Ca2+-dependent contractility through sympathetic nervous system activation. Pathological hemodynamic stress, such as hypertension, is persistent and requires sustained increases in cardiac function. Over time, this causes left […]
|Overexpression of heat shock protein 27 protects against ischaemia/reperfusion-induced cardiac dysfunction via stabilization of troponin I and T.|| Calcium and Contractility|
Overexpression of heat shock protein 27 protects against ischaemia/reperfusion-induced cardiac dysfunction via stabilization of troponin I and T. Lu XY, Chen L, Cai XL, Yang HT Abstract: Aims: Heat shock protein 27 (Hsp27) renders cardioprotection from ischaemia/reperfusion (I/R) injury, but little is known about its role in myofilaments. We proposed that increased expression of Hsp27 may improve post-ischaemic contractile dysfunction by preventing I/R-induced cardiac troponin I (cTnI) and troponin T […]
|Chronic inutero cyclooxygenase inhibition alters PGE2-regulated ductus arteriosuscontractile pathways and prevents postnatal closure.|| Blood Vessel|
NOTE: In the ductus arteriosus of fetal mice: (this is one of the first papers to ever use pressurized myography to study the mouse ductus, and is particularly cool for studying premature fetal vessels). Although prostaglandin E2 (PGE2) vasodilates the ductus arteriosus, tocolysis with cyclooxygenase (COX) inhibitors delays postnatal ductus arteriosus closure. We used fetal mice and sheep to determine whether PGE2 has a role in the development of ductus […]
|High blood pressure arising from a defect in vascular function|
Hypertension, a major cardiovascular risk factor and cause of mortality worldwide, is thought to arise from primary renal abnormalities. However, the etiology of most cases of hypertension remains unexplained. Vascular tone, an important determinant of blood pressure, is regulated by nitric oxide, which causes vascular relaxation by increasing intracellular cGMP and activating cGMP-dependent protein kinase I (PKGI). Here we show that mice with a selective mutation in the N-terminal protein […]
|Sulfonylurea Receptor-Dependent and -Independent Pathways Mediate Vasodilation Induced by ATP-Sensitive K+ Channel Openers|| Blood Vessel|
Sulfonylurea Receptor-Dependent and -Independent Pathways Mediate Vasodilation Induced by ATP-Sensitive K+ Channel Openers Adebowale Adebiyi, Elizabeth M. McNally and Jonathan H. Jaggar Abstract: ATP-sensitive K+ (KATP) channel openers are vasodilators that activate both plasma membrane and mitochondrial KATP channels. Here, we investigated the molecular mechanisms by which diazoxide and pinacidil induce vasodilation by studying diameter regulation of wild-type (SUR2+/+) and sulfonylurea receptor 2 deficient (SUR2-/-) mouse myogenic mesenteric arteries. Ryanodine […]
|Regulation of the fetal mouse ductus arteriosus is dependent oninteraction of nitric oxide and COX enzymes in the ductal wall.|| Blood Vessel|
|Pim-1 Kinase Antagonizes Aspects Of Myocardial Hypertrophy And Compensation To Pathological Pressure Overload.|| Calcium and Contractility||Publication Reviews|
Muraski JA, Fischer KM, Wu W, Cottage CT, Quijada P, Mason M, Din S, Gude N, Alvarez R Jr, Rota M, Kajstura J, Wang Z, Schaefer E, Chen X, MacDonnel S, Magnuson N, Houser SR, Anversa P, Sussman MA
|Mitochondrial matrix metalloproteinase activation decreases myocyte contractility in hyperhomocysteinemia|| Calcium and Contractility||Publication Reviews||µStep Light Source|
Moshal, K. S., Tipparaju, S.M., Vacek, T. P., Kumar, M., Singh, M., Frank, I.E., Patibandla, P.K., Tyagi, N., Rai, J., Metreveli, N., Rodriguez, W.E., Tseng, M.T., and Tyagi, S.C. American Journal of Physiology-Heart and Circulatory Physiology,295(2)
|Mechano-regulated tenascin-C orchestrates muscle repair.||Publication Reviews||MyoPacer Cell Stimulator|
Fluck, M., Mund, S. I., Schittny, J. C., Klossner, S., Durieux, A., & Giraud, M. (2008). Proceedings of the National Academy of Sciences,105(36), 13662-13667
|Maturation of intracellular calcium homeostasis in sheep pulmonary arterial smooth muscle cells.||Publication Reviews||HyperSwitch Light Source|
Goyal, R., Creel, K. D., Chavis, E., Smith, G. D., Longo, L. D., & Wilson, S. M. (2008). American Journal of Physiology-Lung Cellular and Molecular Physiology, 295(5)
|Leptin Attenuates Cardiac Contraction In Rat Ventricular Myocytes. Role of NO.|| Calcium and Contractility||Publication Reviews||PMT Sub-System, µStep Light Source|
Nickola, M. W., Wold, L. E., Colligan, P. B., Wang, G., Samson, W. K., & Ren, J. (2000). Hypertension, 36(4), 501-505
|Late exercise training improves non-uniformity of transmural myocardial function in rats with ischaemic heart failure.|| Calcium and Contractility||Publication Reviews||µStep Light Source|
Mou, Y. A., Reboul, C., Andre, L., Lacampagne, A., & Cazorla, O. (2008). Cardiovascular Research, 81(3), 555-564
|Heat shock protein upregulation protects against pacing-induced myolysis in HL-1 atrial myocytes and in human atrial fibrillation.|| Culture Pacing||Publication Reviews||C-Dish|
Brundel, B. J., Henning, R. H., Ke, L., Gelder, I. C., Crijns, H. J., & Kampinga, H. H. (2006). Journal of Molecular and Cellular Cardiology, 41(3), 555-562
|Electrostimulation induces cardiomyocyte predifferentiation of fibroblasts||Publication Reviews||C-Dish, C-Pace EM|
Genovese, J. A., Spadaccio, C., Langer, J., Habe, J., Jackson, J., & Patel, A. N. (2008). Biochemical and Biophysical Research Communications, 370(3), 450-455
|Electric pulse stimulation induces NMDA glutamate receptor mRNA in NIH3T3 mouse fibroblasts.||Publication Reviews||C-Dish|
Okutsu, S., Hatakeyama, H., Kanazaki, M., Tsubokawa, H., & Nagatomi, R. (2008). The Tohoku Journal of Experimental Medicine, 215(2), 181-187
|Effects of clenbuterol on contractility and Ca2+ homeostasis of isolated rat ventricular myocytes.|| Calcium and Contractility||Publication Reviews||HyperSwitch Light Source|
Siedlecka, U., Arora, M., Kolettis, T., Soppa, G. K., Lee, J., Stagg, M. A., Harding, S.E., Yacoub, M.H., and Terracciano, C. M.N. (2008). American Journal of Physiology-Heart and Circulatory Physiology, 295(5)
|Ecstasy produces left ventricular dysfunction and oxidative stress in rats.||Publication Reviews||µStep Light Source|
Shenouda, S. K., Lord, K. C., Mcilwain, E., Lucchesi, P. A., & Varner, K. J. (2008). Cardiovascular Research, 79(4), 662-670
|Contractile C2C12 myotube model for studying exercise-inducible responses in skeletal muscle.|| Culture Pacing||Publication Reviews||C-Dish, C-Pace EM|
Nedachi, T., Fujita, H., & Kanzaki, M. (2008). American Journal of Physiology-Endocrinology and Metabolism, 295(5)
|Cell volume control in phospholemman (PLM) knockout mice: do cardiac myocytes demonstrate a regulatory volume decrease and is this influenced by deletion of PLM?||Publication Reviews|
Bell, J. R., Lloyd, D., Curl, C. L., Delbridge, L. M., & Shattock, M. J. (2009). Experimental Physiology, 94(3), 330-343
|Atrial Cardiomyocyte Tachycardia Alters Cardiac Fibroblast Function: A Novel Consideration In Atrial Remodeling||Publication Reviews||C-Dish|
Burstein, B., Qi, X., Yeh, Y., Calderone, A., & Nattel, S. (2007). Cardiovascular Research, 76(3), 442-452
|Adenosine A2A and beta-adrenergic calcium transient and contractile responses in rat ventricular myocytes.|| Calcium and Contractility||Publication Reviews||HyperSwitch Light Source|
Dobson, J. G., Shea, L. G., & Fenton, R. A. (2008). American Journal of Physiology-Heart and Circulatory Physiology, 295(6)
|Action potential characterization in intact mouse heart: steady-state cycle length dependence and electrical restitution.||Publication Reviews||HyperSwitch Light Source|
Knollmann, B. C., Schober, T., Petersen, A. O., Sirenko, S. G., & Franz, M. R. (2007). American Journal of Physiology-Heart and Circulatory Physiology, 292(1)
|Actin cytoskeletal modulation of pressure-induced depolarization and Ca(2+) influx in cerebral arteries.|| Blood Vessel||Publication Reviews||HyperSwitch Light Source|
Gokina, N. I., & Osol, G. (2002). American Journal of Physiology-Heart and Circulatory Physiology, 282(4)
|Accelerated de novo sarcomere assembly by electric pulse stimulation in C2C12 myotubes.|| Culture Pacing||Publication Reviews||C-Dish|
Fujita, H., Nedachi, T., & Kanzaki, M. (2007). Experimental Cell Research, 313(9), 1853-1865
|Abnormal Motility in Patients With Ulcerative Colitis: The Role of Inflammatory Cytokines||Publication Reviews||HyperSwitch Light Source|
Vrees, M. D., Pricolo, V. E., Potenti, F. M., & Cao, W. (2002). Archives of Surgery, 137(4)
|Effects of sarcoplasmic reticulum Ca2+-ATPase overexpression in postinfarction rat myocytes.|| Culture Pacing||Publication Reviews||C-Dish|
Ahlers, B. A., Song, J., Wang, J., Zhang, X., Carl, L. L., Tadros, G. M., Rothblum, L.I., and Cheung, J. Y. (2005). Journal of Applied Physiology, 98(6), 2169-2176