Cardiac Tissue Slices: Preparation, Data Acquisition, and Analysis

Date: October 05, 2022
Time: 11:00am EDT

Dr. Bradley Palmer demonstrates the challenging preparation of cardiac tissue slices and measurement of force and calcium.

Unlike isolated cardiomyocytes, cardiac tissue slices better preserve the native myocardial tissue, including both fibroblasts and myocytes. And unlike whole heart studies, slices are better suited for acquisition of functional parameters like calcium transients. Unlike both models, however, cardiac slices have not been widely utilized and their preparation remains a significant challenge for many labs. In this webinar, Dr. Bradley Palmer shows how to successfully prepare cardiac slices for measurements of force and calcium, as well as work loops and stress and strain. Data analysis and interpretation will also be demonstrated.

Key Topics Include:

  • How to successfully prepare cardiac slices
  • What types of data can be acquired from cardiac slices
  • How to analyze and interpret data from cardiac slices

This webinar is part of the Cardiovascular Connections: An Integrative Approach series of webinars presented by InsideScientific, The American Physiology Society, and European Council for Cardiovascular Research. Follow the link below to register for the series, including access to upcoming live webinars and previous webinars on demand.

About the speaker:

Bradley Palmer, PhD

R&D Engineer / Assistant Professor
Molecular Physiology and Biophysics
IonOptix / University of Vermont

Dr. Bradley Palmer’s professional activities have focused on original research demonstrating the molecular basis of cardiac muscle relaxation function. His focus is on two areas: myofilament basis of muscle dynamic stiffness and zinc-dependent alterations in calcium regulation.

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