When combined with the IonOptix Calcium and Contractility System, fluorescence of calcium-sensitive dyes, such as Fluo-4, Fura-2 and/or Indo-1, can be detected using IonWizard, which permits control of experiment acquisition parameters and analysis of data.
Similar to pressure-volume work loops in whole heart, mechanical work loops generated by cardiac slices (displayed in real-time) can be acquired using force-feedback length control. Force-length work loops can be used to derive important functional data including end-systolic and end-diastolic force-length relationships (ESFLR and EDFLR) characterizing the elastance and compliance of the tissue and mechanical work and power.
The cardiac slice preparation technique has been recently championed by Professor Cesare Terracciano (Imperial College London) and his laboratory, including Dr. Fotios Pitoulis. We would like to acknowledge and thank them for their assistance with the tissue preparation.
As with all of our systems, we stand behind our products. When we install our complete systems we use your preparations to help get you started as quickly as possible. And when you need assistance we offer unlimited phone and email support for the lifetime of your system.