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This protocol describes the isolation of primary adult mouse cardiomyocytes using sequential calcium depletion, enzymatic digestion, and gradual calcium reintroduction. The approach is adapted from the method of Matthew Ackers-Johnson et al.
Action Potential Measurements in iPSC-derived and Primary Cardiomyocytes
CytoSolver FAQs: CytoMotion Contractility Module
CytoSolver FAQ: Edge Detection of 3D Cultures
CytoSolver Desktop and Cloud 3.0 User Manual
A video comparing IonOptix contractility algorithms for primary and cultured cardiomyocytes.
A video tutorial describing data fitting of IonOptix calcium and contractility data.
CytoSolver Desktop and Cloud 3.0 User Manual
Calcium and contractility best practices
