Over the last decade human induced pluripotent stem cells (hiPSCs), as well as human embryonic stem cells (hESCs), have emerged as a model system complementary to classic approaches. These cells offer important advantages over typical adult ventricular cardiomyocytes, the most significant of which owing to their human genetic background. Despite their advantages, they are less mature, more amorphous, and provide poorer contrast. To broaden the marketability of our products, we have developed contrast-based algorithms within IonWizard, collectively called CytoMotion, to acquire contractility data from immature cells that lack refined structure and precise features.