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Sarcomere integrated biosensor detects myofilament-activating ligands in real time during twitch contractions in live cardiac muscle

In this article published in the Journal of Molecular and Cellular Cardiology, Vetter and Martin et al. from the University of Minnesota develop and demonstrate a novel troponin C FRET-based biosensor to track thin filament activation in isolated cardiomyocytes and cardiac tissue (papillary muscle) using an IonOptix MyoClamp workstation comprised of our Intact Muscle Chamber atop a Calcium and Contractility System. The authors employed a battery of tests that validate the TnC sensor and use it to demonstrate, for the first time, real-time detection of activation-dependent changes in cardiac troponin C conformation.

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Vetter AD, Martin AA, Thompson BR, Thomas DD, Metzger JM (2020). Sarcomere integrated biosensor detects myofilament-activating ligands in real time during twitch contractions in live cardiac muscle. J Mol Cell Cardiol. 2020 Oct;147:49-61. doi: 10.1016/j.yjmcc.2020.07.012.