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Fu et al investigate the role caveolae play in regulating stretch-mediated βAR-signaling in adult rat ventricular cardiomyocytes. Using the CytoCypher MultiCell, the authors found that hypoosmotic swelling increased cardiomyocyte contractility through β2 but not β1 adrenergic receptor signaling. They also found that this response requires functional caveolae, as evidenced by diminution of the response following MβCD-mediated caveolae disruption. The authors then looked at cAMP release after mechanical stretch in trabeculae from wildtype and Cav3KO mutant mice. While they saw an increase in β2AR-induced cAMP release after stretching in WT trabeculae, they did not see a similar increase in Cav3KO trabeculae. This phenomenon was found in β2AR- but not β1AR-induced cAMP release, further supporting their hypothesis that stretch-mediated increases in contractility occurs via β2 and not β1 adrenergic receptor signaling pathways.

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Fu J, Mansfield C, Diakonov I, Judina A, Delahaye M, Bhogal N, Sanchez-Alonso JL, Kamp T, Gorelik J. Stretch regulation of β2-Adrenoceptor signalling in cardiomyocytes requires caveolae. Cardiovasc Res. 2025 Apr 29;121(3):440-453. doi: 10.1093/cvr/cvae265.