New technology now provides investigators the ability to reliably attach myocytes, mechanically stretch them, make direct force measurements and control cell length intelligently. This webinar reviews best-practices and techniques for attaching, stretching, and studying isolated cells.

A special webinar for basic cardiovascular researchers interested in a novel technique for measuring work output and replicating the four phases of the cardiac cycle at the single cell level.

Ronglih Liao and Davor Pavlovic discuss techniques for isolating myocytes using both traditional and Langendorff-free methodologies.

Misior, A. M., Deshpande, D. A., Loza, M. J., Pascual, R. M., Hipp, J. D., & Penn, R. B. (2009). American Journal of Respiratory Cell and Molecular Biology, 41(1), 24-39

Michiel Helmes, PhD presents new technology and methodology for fast data acquisition and functional analysis of cardiac myocytes, leading to better lab practices and data interpretation.

Orfanos, Z., Gödderz, M. P., Soroka, E., Gödderz, T., Rumyantseva, A., van der Ven, P.F.M, Hawke, T.J., Fürst, D. O. (2016). Scientific Reports, 6(1)

Myocyte contractile activity modulates norepinephrine cytotoxicity and survival effects of neuregulin-1beta. Kuramochi Y1, Lim CC, Guo X, Colucci WS, Liao R, Sawyer DB Abstract: The [...]

The transcription factor NFAT (nuclear factor of activated T-cells) is implicated in cardiac hypertrophy and vasculogenesis. NFAT activation, reflecting dephosphorylation by the calcium-dependent phosphatase, calcineurin, [...]

Rapid electrical stimulation induces early activation of kinase signal transduction pathways and apoptosis in adult rat ventricular myocytes. Kuramochi Y1, Guo X, Sawyer DB, Lim [...]

Induction of heat shock response protects the heart against atrial fibrillation. Brundel BJ, Shiroshita-Takeshita A, Qi X, Yeh YH, Chartier D, van Gelder IC, Henning [...]

Myogenic behavior, prevalent in resistance arteries and arterioles, involves arterial constriction in response to intravascular pressure. This process is often studied in vitro by using [...]

Overexpression of Na+/Ca2+ exchanger alters contractility and SR Ca2+ content in adult rat myocytes. Zhang XQ, Song J, Rothblum LI, Lun M, Wang X, Ding [...]

The control of Ca2+ influx and NFATc3 signaling in arterial smooth muscle during hypertension. Nieves-Cintrón M, Amberg GC, Navedo MF, Molkentin JD, Santana LF Abstract: [...]

High-fat Diet-induced Obesity Leads to Resistance to Leptin-induced Cardiomyocyte Contractile Response. Jun Ren, Bang-Hao Zhu, David P. Relling, Lucy B. Esberg, Asli F. Ceylan-Isik Abstract: [...]

Overexpression of heat shock protein 27 protects against ischaemia/reperfusion-induced cardiac dysfunction via stabilization of troponin I and T. Lu XY, Chen L, Cai XL, Yang [...]

Phosphorylation of phospholamban at threonine-17 reduces cardiac adrenergic contractile responsiveness in chronic pressure overload-induced hypertrophy. Mills GD, Kubo H, Harris DM, Berretta RM, Piacentino V [...]

Novel role of calpain-3 in the triad-associated protein complex regulating calcium release in skeletal muscle. Kramerova I, Kudryashova E, Wu B, Ottenheijm C, Granzier H, [...]

Reactive oxygen species mediate RhoA/Rho kinase-induced Ca2+ sensitization in pulmonary vascular smooth muscle following chronic hypoxia. Jernigan NL, Walker BR, Resta TC Abstract: Recent evidence [...]

Role of the transcription factor sox4 in insulin secretion and impaired glucose tolerance. Goldsworthy M, Hugill A, Freeman H, Horner E, Shimomura K, Bogani D, [...]