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This protocol describes the isolation of primary adult mouse cardiomyocytes using sequential calcium depletion, enzymatic digestion, and gradual calcium reintroduction. The approach is adapted from the method of Matthew Ackers-Johnson et al.
CytoSolver FAQ: Edge Detection of 3D Cultures
A video comparing IonOptix contractility algorithms for primary and cultured cardiomyocytes.
A video tutorial highlighting murine cardiomyocyte isolation protocol and techniques.
A video tutorial highlighting experiment design in IonWizard.
A video tutorial highlighting best practices for data acquisition of calcium and contractility data.
A simple video tutorial highlighting analysis of calcium and contractility data using IonWizard.
A simple video tutorial highlighting analysis of calcium and contractility data using IonWizard.
A simple video tutorial highlighting analysis of calcium and contractility data using IonWizard.
Fang, C. X., Dong, F., Thomas, D. P., Ma, H., He, L., & Ren, J. (2008). American Journal of Physiology-Heart and Circulatory Physiology, 295(3)
