CytoMotion Software Over the last decade human induced pluripotent stem cells (hiPSCs), as well as human embryonic stem cells (hESCs), have emerged as a model system complementary to classic approaches, suitable for cardiac disease modelling. These stem cells offer important advantages, the most significant of which owing to their human genetic background. Despite their advantages, they are less mature, more amorphous, and provide poorer contrast. To facilitate measurement [...]

IonOptix has expanded the functionality of its IonWizard core acquisition software to include increased hardware control as well as a new software module that improves the breadth of the MyoStretcher myocyte stretch and force measurement system, an integrated, full featured system that enables scientists to investigate muscle mechanics at the single cell level while synchronously recording quantitative fluorescence and sarcomere length. The MyoStretcher has been engineered to attach single myocytes and [...]

The MultiCell Automated Cell Finder enables users to load a sample, walk away, and come back to as many as hundreds of measured cells. In addition to ease of use, Automated Cell Finder eliminates human bias in cell selection. Specify the following criteria for cell selection: Cell area and aspect ratio Distance between cells Motion pattern when scanning for cells Cell motion detection The software will scan the field [...]

The new C-Pace Navigator software allows users to operate both the C-Dish and C-Stretch with a user-friendly interface, making it easy to customize stretch and stimulation protocols. Simple adjustments of both stretch and electrical parameters for each channel Pre-defined basic protocols for simple pacing procedures Adjustable trapezoidal waveform with expansion, hold, and contraction phases Save and re-load stretch and stimulation protocols [...]

The CyFlo software, when coupled with our custom CytoCypher perfusion system, gives users an easy-to-use and precise method of perfusion and compound delivery. In CyFlo, configure two independehttps://www.ionoptix.com/products/componentnt in-flow pumps to deliver the number of solutions to the number of wells desired. An easy priming procedure ensures that only solution and not air is delivered to your sample. Choose to either add, remove, or perfuse with balanced flow a [...]

CytoSolver provides fast automatic data analysis. Analyze batches of data in seconds by simply uploading files and letting CytoSolver take care of automatic transient rejection, data averaging, and curve fitting. Customize rejection parameters to suit your research. Available as a desktop or cloud-based platform. Cloud-based CytoSolver includes data visualization for quick box plots and bar graphs. Whether you're using MultiCell or MultiCell Lite to analyze hundreds of cells at [...]

IonOptix’ FloAcq acquisition and recording module provides real-time indicators of arterial flow dynamics. The endothelial response to flow-induced stress (e.g., the generation and secretion of nitric oxide) is an important modulator of vascular tone and function, linking vasodilation and constriction to the dynamics of fluid flow. IonWizard’s FloAcq acquisition module is the first to perform real-time calculations of important indicators of arterial flow, including shear stress. By acquiring the [...]

MultiAcq gives you the speed and ease of microscope control needed to achieve high throughput in your experiments. With this new interface, you can: Focus on your sample, either manually or automatically Quickly move around the dish using buttons or keyboard shortcuts for maximum speed Digitally rotate, saving time and effort Mark cell position and rotation for repeated measurements [...]

IonWizard integrates the presentation of fluorescence traces and images, analog voltages, and cell length data under an intuitive interface. It analyzes raw data by performing ratio, ion, or linear calibration calculations. Fluorescence calculations include a comprehensive selection of background subtraction techniques.  Specialized functions such as trace averaging, composite images, and transient analysis eliminate common extra work. Clipboarding and exporting of trace and image data eases integration with other programs. [...]

The cardiac work cycle consists of four well-defined isometric and isotonic phases. The IonOptix MultiClamp software enables fast feedback control of pre- and after-load force levels. Together with the IonOptix MyoStretcher OptiForce force transducer and fast piezo motor, we can modulate the force development by rapidly changing the length of the myocyte in response to changing force load. By clamping the diastolic and systolic force levels (pre- and after-load), [...]