The MultiCell High Throughput System is an automated, real-time, high-content data acquisition and analysis solution.
The MultiCell HTS system offers high-speed, high-content myocyte contractility and fluorescence data acquisition from hundreds of myocytes per hour by using our automated cell finding image analysis. The system includes a fast x-y-z position programmable scanning microscope which can mark and find position, focus, and orientation of isolated myocytes enabling repeated measures of contraction-relaxation function. The system also enables identification and real-time characterization of iPSC-derived cardiomyocytes using our CytoMotion algorithm. The enclosed microscope maintains stable temperature while allowing full access for introduction of perfusion as well as electrical stimulation leads.
While studies utilizing IonOptix Calcium and Contractility systems have proven extremely valuable for many years, higher throughput is required to accommodate faster drug discovery and a growing motivation in basic research toward rapid, large scale data collection and analysis. Isolated myocytes display a high degree of functional variability, which can result in poor characterization of myocyte dynamics. And iPSC-CM contractile function can vary from batch to batch as well as over differing developmental periods. Acquiring data from greater numbers of myocytes provides the statistical power to characterize myocyte function with better accuracy and confidence. This is a significant enhancement in phenotype quantification as it expedites assessment of drug efficacy/toxicity and greatly reduces costs by eliminating dangerous or marginal compounds.
Every technical detail of the MultiCell HTS has been thoroughly considered to maintain the highest level of precision. Batch analysis enables fast, automated characterization from hundreds of cells, while still characterizing all of the same important parameters found in IonWizard’s core analysis. Double exponential fitting improves data fitting of calcium re-uptake and subsequent tau calculations, an important parameter used to describe sodium-calcium exchange and SERCA activity. MultiCell HTS offers the same thorough acquisition and analysis that our users expect from an IonOptix system at much higher acquisition and analysis rates.
Our optional MultiWell stage adds even more functionality to the system. The X/Y stage is integrated into our Cell Finder module to allow programmable travel from well to well, improving the system’s capability and usability. Pre-programmed well maps are available within the software for various tissue culture plates, including 6-well, 24-well, 96-well, and more.
We stand behind every product we sell, and we are committed to ensuring you get the most out of your IonOptix system. Our goal is to help you collect meaningful, reliable data as quickly as possible. Whether you need help with your system or have questions about the application, we’re here for you.
FEATURES
COMPONENTS
MultiCell High Throughput Systems can be configured to meet your specific needs. Excitation light sources and optics are customized to any fluorescent probe, and systems can include balanced flow perfusion and multi-well or single-well dishes depending on your application. With the MyoCam-S3, square pixels allow for digital rotation and an sCMOS sensor yields high frame rates.
BROWSE THE TABS to learn more about the components that complete this system.
Choose between the enclosed CytoCypher microscope for the full MultiCell system, or an inverted microscope for the MultiCell Lite.
The fluorescence excitation light source is chosen with your specific research needs in mind and can be configured for almost any dye or combination of dyes. Options include ultra-fast, monochromatic LEDs. LEARN MORE
The sensors within MultiCell systems are responsible for translating cellular signal into data. Cell contractility is measured using the high-speed MyoCam-S3 digital camera, and whole-cell fluorescence photometry is measured with a photomultiplier tube (PMT). LEARN MORE
The field stimulator provides acute stimulation of cells and is an important component of excitation-contraction studies. Use the MyoPacer for simple stimulation protocols or the MyoPacer EP for advanced sequencing and arrhythmic stimulation. The StimSwitch multiplexer can be combined with either stimulator for multiwell plates. LEARN MORE
The FSI ties the MultiCell system components together by communicating between hardware and the computer, allowing for synchronous control of contractility and/or calcium measurements as well as inputs and outputs for external devices. LEARN MORE
A variety of dish types and stimulation/perfusion lids can be used, in combination with the CytoCypher Balanced Flow Perfusion System. LEARN MORE
SOFTWARE
IonWizard seamlessly integrates the familiar calcium and contractility trace recording with new modules for MultiCell acquisition and automatic cell finding and recording.
With IonWizard 7 you can perform advanced sequencing functionality, allowing you to set up MultiCell and regular experiments by creating epoch loops. Also, in the event of a software crash, cell positions are saved and data files can be recovered.
In addition to IonWizard, software modules are available with the MultiCell system to create a turn-key, high-throughput system.
