This document shows in steps how to do a standard analysis with IonWizard 5 & 6. We have also added an example data file that you can use to practice the tutorial with.

The endothelial response to flow-induced stress (e.g., the generation and secretion of nitric oxide) is an important modulator of vascular tone and function, linking vasodilation and constriction to …

The largest group of IonOptix users are investigators who study cardiac myocytes and want information about contractility parameters and the associated calcium transients. Our aim is to offer you a complete recording system for these experiments.

Chronic electrical stimulation has been shown to prevent the dedifferentiation of myocytes that occurs in long term culture...

5 years of the C-Pace: a short review Cardiac tissue * Skeletal muscle * Stem cells * Cell-lines * Cardiogenic Fibroblasts * Neuronal fibroblasts * Osteoblasts It has been more than 5 years …

For dual excitation measurements with Fura-2, we offer two methods of collecting data, interleaved sampling and the interpolated numerator (or denominator) method.

Introduction This is a very, very basic introduction into the theory of fluorescence measurements and its practical implications as applied to experiments done using IonOptix equipment. For a thorough …

Many different protocols exist for loading cells with dye, due to diffences between cell types in how they treat Fura. Below a protocol is provided that works well for freshly isolated cardiac …

Overview Fluorescence signal quality is directly proportional to fluorescence light intensity (which is proportional to excitation light intensity, fluorescence yield of the fluorophore, and light …

Engineered skeletal muscle from electrically stimulated myoblast cells Check out this video from researchers at the Department of Biomedical Engineering, Eindhoven University of Technology.

Check out this video from researchers at the Department of Internal Medicine B, University Medicine Greifswald.

Interest in the physiology and pathology of the circulatory system has led to a substantial increase in the number of laboratories studying isolated and pressurized blood vessels.

Raake, P. W., Vinge, L. E., Gao, E., Boucher, M., Rengo, G., Chen, X., DeGeorge Jr., B.R., Matkovich, S., Houser, S.R., Most, P., Eckhart, A.D., Dornll, G.W., and Koch, W. J. (2008). Circulation Research, 103(4), 413-422

Kroll, K., Chabria, M., Wang, K., Häusermann, F., Schuler, F., & Polonchuk, L. (2017). Progress in Biophysics and Molecular Biology, 130, 212-222

New technology now provides investigators the ability to reliably attach myocytes, mechanically stretch them, make direct force measurements and control cell length intelligently. This webinar reviews best-practices and techniques for attaching, stretching, and studying isolated cells.

A special webinar for basic cardiovascular researchers interested in a novel technique for measuring work output and replicating the four phases of the cardiac cycle at the single cell level.

Ronglih Liao and Davor Pavlovic discuss techniques for isolating myocytes using both traditional and Langendorff-free methodologies.

Misior, A. M., Deshpande, D. A., Loza, M. J., Pascual, R. M., Hipp, J. D., & Penn, R. B. (2009). American Journal of Respiratory Cell and Molecular Biology, 41(1), 24-39

Michiel Helmes, PhD presents new technology and methodology for fast data acquisition and functional analysis of cardiac myocytes, leading to better lab practices and data interpretation.

Orfanos, Z., Gödderz, M. P., Soroka, E., Gödderz, T., Rumyantseva, A., van der Ven, P.F.M, Hawke, T.J., Fürst, D. O. (2016). Scientific Reports, 6(1)