Athlin S, Steger N, Thazhath-Veettil J, Gentile C, Demirel I, Sirsjö A, Kienesberger PC, Nordenskjöld A, Paramel GV. Cardiac spheroids as a human model for inflammation induced cardiac dysfunction. Biofabrication. 2026 Mar 11;18(2). doi: 10.1088/1758-5090/ae4ad7.
RNA-binding protein SAMD4A targets FGF2 to regulate cardiomyocyte lineage specification from human embryonic stem cells (2025)
ionoptix2026-06-16T18:15:23-05:00Yi N, Wang HR, Zhu YP, Xiao T, Lin Q, Liu H, Meng YL, Sun YZ, Lin F, Hu SY, Cao HM, Zhang JF, Peng LY, Li L. RNA-binding protein SAMD4A targets FGF2 to regulate cardiomyocyte lineage specification from human embryonic stem cells. Stem Cell Res Ther. 2025 Mar 18;16(1):144. doi: 10.1186/s13287-025-04269-7.
Mutation-induced LZTR1 polymerization provokes cardiac pathology in recessive Noonan syndrome (2024)
ionoptix2026-06-16T18:55:06-05:00Busley AV, Gutiérrez-Gutiérrez Ó, Hammer E, Koitka F, Mirzaiebadizi A, Steinegger M, Pape C, Böhmer L, Schroeder H, Kleinsorge M, Engler M, Cirstea IC, Gremer L, Willbold D, Altmüller J, Marbach F, Hasenfuss G, Zimmermann WH, Ahmadian MR, Wollnik B, Cyganek L. Mutation-induced LZTR1 polymerization provokes cardiac pathology in recessive Noonan syndrome. Cell Rep. 2024 Jul 23;43(7):114448. doi: 10.1016/j.celrep.2024.114448.
Modified Langendorff-free Cardiomyocyte Isolation
ionoptix2025-08-18T18:04:20-05:00This protocol describes the isolation of primary adult mouse cardiomyocytes using sequential calcium depletion, enzymatic digestion, and gradual calcium reintroduction. The approach is adapted from the method of Matthew Ackers-Johnson et al.
AP Measurements: iPSC- vs primary cardiomyocytes
ionoptix2025-05-14T18:46:59-05:00Action Potential Measurements in iPSC-derived and Primary Cardiomyocytes
Calcium Indicator Dye Loading in Cardiac Slices
ionoptix2026-02-04T12:46:43-05:00Calcium Indicator Dye Loading in Cardiac Slices
DataVis Frequently Asked Questions
ionoptix2023-12-04T18:34:08-05:00CytoSolver Desktop and Cloud 3.0 User Manual
CytoSolver Desktop and Cloud 3.0 User Manual
ionoptix2023-08-03T18:46:25-05:00CytoSolver Desktop and Cloud 3.0 User Manual
Calcium and contractility best practices
ionoptix2023-12-01T17:42:51-05:00Calcium and contractility best practices
CytoSolver 3.0 Batch Analysis Guide
ionoptix2023-12-01T17:42:06-05:00CytoSolver 3.0 Batch Analysis Guide
CytoSolver 3 analysis of paced cardiomyocytes
ionoptix2023-12-01T17:41:04-05:00Analyzing Ca & Contractility data from stimulated cardiomyocytes
CytoSolver 3 analysis of unpaced iPSC-CM
ionoptix2023-12-01T17:39:36-05:00Analyzing iPSC-CM Ca & Contractility data
Functional Characterization of Excitation-Contraction Coupling in Cardiomyocytes
ionoptix2022-09-14T17:48:55-05:00Dr. Joe Soughayer and Dr. Adam Veteto provide an in-depth overview of excitation-contraction (E-C) coupling in cardiomyocytes and the tools to characterize this.
Excitation-Contraction Coupling: Simultaneous Measures of Intracellular Calcium & Action Potentials
ionoptix2022-09-14T17:41:27-05:00Patrick Schönleitner, PhD and Francisco Altamirano, PhD present their work involving simultaneous measurements of intracellular calcium, membrane voltage, and contractility of cardiomyocytes.
Measuring iPSC-CM Calcium & Contractility
ionoptix2023-01-31T14:41:13-05:00Measuring hiPSC-CM Ca & Contractility
iPSC-derived Cardiomyocyte Contractility Acquisition and Analysis Demo
ionoptix2022-08-09T17:46:40-05:00Understand how to acquire and analyze contractility data from iPSC-derived cardiomyocytes.
A How-To Demonstration of Higher Throughput Excitation-Contraction Coupling Investigations
ionoptix2021-08-18T16:41:11-05:00From myocyte isolation to data acquisition, analysis, and post-analysis plotting, Dr. Michiel Helmes and Dr. Diederik Kuster demonstrate best practices and new techniques in high-content, higher throughput investigations of excitation-contraction coupling in isolated cardiomyocytes.
Simultaneous Action Potential, Calcium, and Contractility Case Study
ionoptix2021-01-12T11:42:41-05:00Combined OAP, Ca, and Cont measurements in adult cardiomyocytes
IonOptix Video Tutorials: Murine Cardiomyocyte Isolation
ionoptix2026-02-17T19:58:46-05:00A video tutorial highlighting murine cardiomyocyte isolation protocol and techniques.